This lesson describes the light-independent reactions of photosynthesis as reduction of carbon dioxide using the products of the light-dependent reactions. The detailed PowerPoint and accompanying resources have been designed to cover point 5.8 (i) of the Pearson Edexcel A-level Biology A (Salters-Nuffield) specification and therefore describes carbon fixation in the Calvin cycle and the roles of GP, GALP, RuBP and RUBISCO).
The lesson begins with an existing knowledge check where the students are challenged to recall the names of structures, substances and reactions from the light-dependent stage in order to reveal the abbreviations of the main 3 substances in the light-independent stage. This immediately introduces RuBP, GP and GALP and students are then shown how these substances fit into the cycle. The main section of the lesson focuses on the three phases of the Calvin cycle and time is taken to explore the key details of each phase and includes:
- The role of RUBISCO in carbon fixation
- The role of the products of the light-dependent stage, ATP and reduced NADP, in the reduction of GP to GALP
- The use of the majority of the GALP in the regeneration of RuBP
A step-by-step guide, with selected questions for the class to consider together, is used to show how 6 turns of the cycle are needed to form the GALP that will then be used to synthesise 1 molecule of glucose. A series of exam-style questions are included at appropriate points of the lesson and this will introduce limiting factors as well as testing their ability to answer questions about this stage when presented with an unfamiliar scientific investigation. The mark schemes are included in the PowerPoint so students can assess their understanding and any misconceptions are immediately addressed.
This lesson has been specifically written to tie in with the previous lessons on the structure of a chloroplast and the light-dependent reactions as well as the upcoming lesson on the products of the light-independent reactions.
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So pleased to have found this author! In previous years the pupils have found this biochemistry quite overwhelming, but last week using this powerpoint and resources they found it.... dare I say.... easy! I assessed them at the end of the week and sure enough they have mastered the LIR. Thank you!
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