This lesson describes how thin-layer chromatography can be used to separate photosynthetic pigments. The PowerPoint and accompanying resource are part of the 2nd lesson in a series of 2 lessons which have been planned to cover point [c] of module 5.2.1 of the OCR A-level biology A specification.
As mentioned above, this lesson has been designed to build and check on knowledge from the previous lesson which covered the importance of the photosynthetic pigments. The lesson begins by challenging them to recall that chromatography is the separation method that has a stationary and a mobile phase and then to realise that the photosynthetic pigments could be separated using this technique. A step-by-step guide goes through the TLC process, and understanding and prior knowledge checks are used throughout to add key details. Moving forwards, the formula for the retention factor is provided, and the students are challenged to apply this formula to recognise the values for the chlorophylls and the carotenoids.
All answers to the understanding and prior knowledge checks are embedded into the PowerPoint to allow students to assess their progress.
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Module 5.2: Photosynthesis & Respiration (OCR A-level Biology A)
Photosynthesis and respiration are two of the most commonly-assessed topics in the terminal A-level exams but are often poorly understood by students. These 16 lessons have been intricately planned to contain a wide range of activities that will engage and motivate the students whilst covering the key detail to try to deepen their understanding and includes exam-style questions so they are prepared for these assessments. The following specification points in modules 5.2.1 and 5.2.2 of the OCR A-level Biology A course are covered by these lessons: * The structure of a chloroplast and the sites of the two main stages of photosynthesis * The importance of the photosynthetic pigments * Using thin-layer chromatography to separate photosynthetic pigments * The light-dependent stage of photosynthesis * The fixation of carbon dioxide and the light-independent stage of photosynthesis * The uses of triose phosphate * Factors affecting photosynthesis * The need for cellular respiration * The structure of the mitochondrion * The process and site of glycolysis * The link reaction and its site in the cell * The process and site of the Krebs cycle * The importance of coenzymes in cellular respiration * The process and site of oxidative phosphorylation * The chemiosmostic theory * The process of anaerobic respiration in eukaryotes * The relative energy values of carbohydrates, lipids and proteins as respiratory substrates * The use of the respiratory quotient Due to the detail of these lessons, it is estimated that it will take in excess of 2 months of A-level lessons to cover this module If you would like to sample the quality of the lessons, download the uses of triose phosphate, link reaction and respiratory substrates lessons as these have been shared for free
Module 5.2.1: Photosynthesis (OCR A-level Biology A)
This lesson bundle contains 7 fully-resourced lessons which will engage and motivate the students whilst covering the content of module 5.2.1 of the OCR-A-level Biology A specification in the depth of detail required to support them to answer assessment questions on this topic of PHOTOSYNTHESIS confidently. This cellular reaction can be poorly understood by students so intricate planning has gone into the design of the lesson PowerPoints and accompanying resources to ensure that key details are embedded and reinforced throughout and that knowledge acquired in previous lessons is regularly checked. The following specification points are covered by the lessons in this bundle: * The structure of the chloroplasts and the two main stages of photosynthesis * The importance of photosynthetic pigments for photosynthesis * Using thin-layer chromatography to separate photosynthetic pigments * The light-dependent stage of photosynthesis * The fixation of carbon dioxide and the light-independent stage of photosynthesis * The uses of triose phosphate * Factors affecting photosynthesis If you would like to sample the quality of the lessons, then download the "uses of triose phosphate" lesson as this has been shared for free
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