This detailed lesson describes how recombinant DNA is produced using restriction endonucleases and DNA ligase and is inserted into other cells. The engaging PowerPoint and accompanying resources have been designed to cover points 8.18 & 8.19 of the Edexcel International A-level Biology specification.
The lesson begins with a definition of genetic engineering and recombinant DNA to allow students to begin to understand how this process involves the transfer of DNA fragments from one species to another. Links are made to the genetic code and transcription and translation mechanisms, which were met in topic 2, in order to explain how the transferred gene can be translated in the transgenic organism. Moving forwards, the method involving reverse transcriptase and DNA polymerase is introduced and their knowledge of the structure of the polynucleotides and the roles of enzymes is challenged through questions and discussion points. Restriction endonucleases are then introduced and time is taken to look at the structure of a restriction site as well as the production of sticky ends due to the staggered cut on the DNA. A series of exam-style questions with displayed mark schemes are used to allow the students to assess their current understanding.
The second half of the lesson looks at the culture of transformed host cells as an in vivo method to amplify DNA fragments. Students will learn that bacterial cells are the most commonly transformed cells so the next task challenges their recall of the structures of these cells so that plasmid DNA can be examined from that point onwards.
Time is taken to explore the finer details of each step such as the addition of the promoter and terminator regions, use of the same restriction enzyme to cut the plasmid as was used to cut the gene and the different types of marker genes.
As well as understanding and prior knowledge checks, quick quiz competitions are used throughout the lesson to introduce key terms such as cDNA and EcoR1 in a fun and hopefully memorable way
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Topic 8: Coordination, Response and Gene Technology (Edexcel Int. A-level biology)
Topic 8 of the Edexcel International A-level biology specification is content heavy and therefore all 11 lessons included in this bundle have been planned to cover this content in an engaging and memorable way. The lessons are filled with a wide variety of tasks, including understanding and prior knowledge checks, guided discussion periods and quick quiz competitions. Answers to all of the knowledge checks are embedded into the PowerPoints to allow the students to assess their progress. The following specification points are covered by this bundle: 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.8, 8.10, 8.13, 8.14, 8.18, 8.19, 8.20 If you would like to get a sense for the quality of the lessons in this bundle, then download the nervous and hormonal control, saltatory conduction and pupil reflex lessons as these have been shared for free.
£18.00
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Topic 8: Coordination, Response and Gene Technology (Edexcel Int. A-level Biology)
This bundle contains 8 fully-resourced lessons which have been designed to cover the following content in topic 8 of the Edexcel International A-level Biology specification: 8.1: Know the structure and function of sensory, relay and motor neurones 8.2: Understand how the nervous system of organisms can cause effectors to respond to a stimulus 8.4: Understand how a nerve impulse is conducted along an axon 8.5: Understand the role of myelination in saltatory conduction 8.6 (i): Know the structure and function of synapses in nerve impulse transmission 8.6 (ii): Understand how the pupil dilates and contracts 8.8: Understand how the nervous system of organisms can detect stimuli with reference to rods in the retina of mammals 8.10: Know that the mammalian nervous system consists of the central and peripheral nervous systems 8.13: Understand how coordination in animals is brought about through nervous and hormonal control 8.18: Understand how recombinant DNA can be produced 8.19: Understand how recombinant DNA can be inserted into other cells Each of the lessons contains a wide range of activities, which include exam-style questions, guided discussion periods and quick quiz competitions, and these will motivate the students whilst the difficult A-level content is covered If you would like to see the quality of lessons included in this bundle then download the pupil reflex, saltatory conduction and nervous and hormonal control lessons as these have been uploaded for free
£10.00
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