This lesson looks at the use of the polymerase chain reaction (PCR) as an in vitro method to amplify DNA fragments as part of the recombinant DNA technology process. The clear PowerPoint has been designed to cover the second part of point 8.4.1 of the AQA A-level Biology specification.
A quick quiz competition is used to introduce the PCR abbreviation before students are encouraged to discuss the identity of the enzyme involved and to recall the action of this enzyme. Students will learn that this reaction involves cyclical heating and cooling to a range of temperatures so the next part of this lesson looks at these particular temperatures so the important parts of each of the steps can be understood. Time is taken to examine the key points in detail, such as the specific DNA polymerase that is used and how it is not denatured at the high temperature as well as the involvement of the primers.
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This fully-resourced lesson bundle covers the content as detailed in topic 8.4.1 of the AQA A-level Biology specification (recombinant DNA technology). All of the lessons have been written to contain a wide range of activities that will maintain student engagement whilst this potentially difficult concept is explained. Links are continuously made to previous lessons in topic 8 as well as those covered in the AS year (mainly topic 4) If you would like to see the quality of the lessons, download the producing DNA fragments lesson as this has been uploaded for free
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