Unlock the secrets of plant genetics with this classroom protocol designed for students as they extract chloroplast DNA from daffodil leaves, preparing it for nanopore sequencing. This resource provides a step-by-step guide that encourages active learning through hands-on experimentation and critical thinking. Part of the DaffDNA collection of materials. Audience Designed for high school and undergraduate students studying biology or genetics Components Detailed written protocol with clear instructions and accompanying photos Questions integrated into each step to enhance understanding and engagement. Discussion points on the role and importance of each chemical and physical step in the extraction process Learning Objectives Understand the process of DNA extraction and the specific techniques used to isolate chloroplast DNA Develop laboratory skills such as centrifugation, use of buffers, and micropipetting Gain insights into the preparation of DNA samples for advanced sequencing technologies like nanopore sequencing Apply critical thinking to interpret the purpose of each step in the protocol and its impact on data quality Classroom Application This resource is perfect for instructors looking to provide students with practical laboratory experience that bridges theoretical knowledge with real-world applications. The protocol includes checkpoints where students can pause and discuss their observations, promoting collaborative learning and deeper comprehension. Keywords: Chloroplast DNA extraction, nanopore sequencing, daffodil, biology education, laboratory protocol, student engagement, hands-on learning, genetics experiment. Notes for Educators: This protocol is designed to be used in a classroom laboratory setting with standard equipment. Educators are encouraged to review the protocol in advance and prepare the necessary materials for an efficient and smooth learning experience. The included questions aim to spark curiosity and discussion, making this resource not just an experiment but a full learning module.