pptx, 1.83 MB
pptx, 1.83 MB
docx, 135.58 KB
docx, 135.58 KB
Investigate the effect of pH on the rate of reaction (RP 5-separate science). This practical was completed in one lesson, students were asked to construct a graph from their data for homework.

How did i deliver this practical?
I used 5 different buffered pH solutions (4,5,6,7,8). I split my class into two groups ( 2 x 15). Within each group there were 5 sub-groups. This allowed me to gather two sets of results for each pH.
Group A Group B
| Student group 1-pH 4 | Student group 1-pH 4
| Student group 2-pH 5 | Student group 2-pH 5
| Student group 3-pH 6 | Student group 3-pH 6
| Student group 4-pH 7 | Student group 4-pH 7
| Student group 5-pH 8 | Student group 5-pH 8

AQA spec link: 4.2.2.1
Relevant chapter: B3 Organisation and the digestive system. AQA Biology third edition textbook-Page 46-47

Students are required to know the following;

Required practical activity 5: investigate the effect of pH on the rate of reaction of amylase enzyme.
Students should use a continuous sampling technique to determine the time taken to completely digest a starch solution at a range of pH values. Iodine reagent is to be used to test for starch every 30 seconds. Temperature must be controlled by use of a water bath or electric heater.
AT skills covered by this practical activity: AT 1, 2, 5 and 8. This practical activity also provides opportunities to develop WS and MS. Details of all skills are given in Key opportunities for skills development.

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