Aseptic techniques/culturing bacteria/testing antibiotics *BIG PRACTICAL*

Resources developed as part of a *BIG PRACTICAL*. These are designed to practice a skill - usually using a preliminary investigation - then a full investigation write up. These are tailored toward the way the new GCSE (2016 on wards) is designed to test practical skills as well as context.

First lesson
Resources: aseptic techniques practice, pupils-account-of-aseptic-techniques (+ppt)
Practical: Pupils culture a known bacterial both using aseptic techniques, with an aim of achieving zero contamination.
Activities order: Teacher intro, practical write up using frame and practical, KILLER CHALLENGE correcting a poor aseptic method

Second lesson
Resources: Testing antibiotics (+ppt)
Practical: Pupils complete the same method of culturing known bacterial broths using aseptic techniques but add antibiotic disks.
Activity order: Look at plates from previous lesson and look if they have been successful and what they need to improve. Practical and write up.

Third lesson
Resources: Antibiotic plate sheet, Diameter-zone-of-inhibition (+ppt)
Activity order: Look at antibiotic plates, Complete sheet looking at antibiotics and comparing zones of inhibition, calculating zones of inhibition for comparison.