Genetic technology (CIE International A-level Biology)

This extensive and fully-resourced lesson describes the steps in the production of recombinant DNA in genetic technology. Both the engaging PowerPoint and accompanying resources have been written to cover all of the detailed content of topic 19.1 of the CIE International A-level Biology specification apart from the polymerase chain reaction and gel electrophoresis as these are found in other uploaded lessons.

The lesson begins with a definition of recombinant DNA to allow students to begin to understand how this process involves the transfer of DNA fragments from one species to another. Links are made to the genetic code and transcription and translation mechanisms, which were met in topic 6, in order to explain how the transferred gene can be translated in the transgenic organism. Moving forwards, the method involving reverse transcriptase and DNA polymerase is introduced and their knowledge of the structure of the polynucleotides and the roles of enzymes is challenged through questions and discussion points. Restriction enzymes are then introduced and time is taken to look at the structure of a restriction site as well as the production of sticky ends due to the staggered cut on the DNA. A series of exam-style questions with displayed mark schemes are used to allow the students to assess their current understanding.
The second half of the lesson looks at the culture of transformed host cells as an in vivo method to amplify DNA fragments. Students will learn that bacterial cells are the most commonly transformed cells so the next task challenges their recall of the structures of these cells so that plasmid DNA can be examined from that point onwards. The following key steps are described and explained:

• Remove and prepare the plasmid to act as a vector
• Insert the DNA fragment into the vector
• Transfer the recombinant plasmid into the host cell
• Identify the cells which have taken up the recombinant plasmid
• Allow the transformed host cells to replicate and express the novel gene

Time is taken to explore the finer details of each step such as the addition of the promoter and terminator regions, use of the same restriction enzyme to cut the plasmid as was used to cut the gene and the different types of marker genes.

As well as understanding and prior knowledge checks, quick quiz competitions are used throughout the lesson to introduce key terms such as cDNA and EcoR1 in a memorable way.

Due to the detail that is included in this lesson, it is estimated that it will take in excess of 3 hours to cover the points