This lesson describes how chloroplast pigments can be separated by chromatography and the pigments identified by Rf values. The PowerPoint and accompanying resource have been planned to cover point 5.7 of the Edexcel International A-level biology specification and to build and check on the knowledge acquired in the previous lesson on the absorption and action spectrum.
The lesson begins with a challenge, where the students must recall that chromatography is the separation method that has a stationary and a mobile phase and then to realise that the chloroplast pigments could be separated using this technique. A step-by-step guide goes through the thin-layer chromatography process, and understanding and prior knowledge checks are used throughout to add key details. Moving forwards, the formula for the retention factor is provided, and the students are challenged to apply this formula to recognise the values for the chlorophylls and the carotenoids to allow them to be identified.
All answers to the understanding and prior knowledge checks are embedded into the PowerPoint to allow students to assess their progress.
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