This lesson describes the principles of cell fractionation and ultracentrifugation as used to separate cell components. The engaging PowerPoint and accompanying resources are part of the final lesson in a series of 4 lessons which have been planned to cover the details of point 2.1.3 of the AQA A-level biology specification.
This lesson begins by informing the students that several of the key terms in this lesson, including the lesson title, end in -ation, and therefore they have to use the clues to work out that the 1st one is cell fractionation. A quiz round like this runs throughout the lesson, introducing homogenisation, filtration and ultracentrifugation in a memorable way. Time is taken to explain each of the processes in detail, and where possible, links are made to previously covered content as well as content that will be met in future lessons. For example, students will learn that the solution must be kept ice-cold and isotonic, and they are challenged to recognise that the low temperature is to reduce the activity of potentially damaging enzymes, before being told that there will be no net movement of water by osmosis because of the isotonic solution. The answers to all understanding and prior knowledge checks are embedded into the PowerPoint to allow students to assess their progress. When explaining the process of ultracentrifugation, the students are given an opportunity to predict which of 6 listed organelles will be found in the 1st pellet because it is the heaviest, right down to the lightest organelle. The lesson finishes with several exam-style questions to check that they’ve understood this separation technique and have a strong knowledge of cells and their organelles.
This lesson has been planned to continously link with the other lessons in topic 2.1 (Cell structure).
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Methods of studying cells (AQA A-level biology)
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Topic 2.1: Cell structure (AQA A-level Biology)
This bundle of 9 lesson PowerPoints and accompanying resources contain a wide variety of tasks which will engage and motivate the students whilst covering the details of topic 2.1 of the AQA A-level Biology specification. Cells and their structure are linked to all of the other 7 topics in this course so a clear understanding is critical to a student's success. The tasks which include exam-style questions (with displayed mark schemes), discussion points and quiz competitions will cover the following parts of topic 2.1: * The structure and function of the cell-surface membrane, nucleus, nucleolus, mitochondria, chloroplasts, Golgi apparatus, lysosomes, ribosomes, RER and SER, cell wall and cell vacuole * The specialised cells of complex, multicellular organisms * The structures of a typical prokaryotic cell * The differences between prokaryotic and eukaryotic cells * The structure of viruses * The principles and limitations of optical microscopes, transmission electron microscopes and scanning electron microscopes * Measuring the size of an object using an optical microscope * Using the magnification formula * The principles of cell fractionation and ultracentrifugation If you would like to sample the quality of these lessons, then download the eukaryotic animal cells, viruses and microscopes lessons as these have been uploaded for free
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