This lesson explains the principles of the polymerase chain reaction (PCR) and the PowerPoint has been designed to cover point 6.1.3 (d) of the OCR A-level Biology A specification
A quick quiz competition is used to introduce the PCR abbreviation before students are encouraged to discuss the possible identity of the enzyme involved and to recall the action of this enzyme. Students will learn that this reaction involves cyclical heating and cooling to a range of temperatures so the next part of this lesson focuses on each temperature and specifically the reasons behind the choice. Time is taken to examine the key points in detail, such as why Taq polymerase has to be used as it is not denatured at the high temperature as well as the involvement of the primers. This process is closely linked to other techniques like electrophoresis which is covered in a later lesson and ties are continuously made throughout the lesson
This process is mentioned in other uploaded lessons in this module such as electrophoresis and genetic engineering to allow students to understand how it is critical for DNA analysis
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