Restriction enzymes - how they work and choosing the right one

A worksheet aimed at advanced biology students who are studying a topic on gene technology and the role of restriction endonucleases. The worksheet consists of task where students are required to choose the most appropriate restriction enzyme to cut out a gene from a piece of DNA. Students are given four different examples of restriction enzymes, together with information about the recognition sequence for each enzyme. Using this information, students must then select the most appropriate enzyme to cut out the gene.

Key words: restriction endonuclease, gene, recognition sequence