This fully-resourced lesson describes the effects of enzyme and substrate concentration on the rate of enzyme-catalysed reactions. The PowerPoint and accompanying resources are the third in a series of 4 lessons which cover the details of point 3.2 (a) of the CIE A-level Biology specification.
The first part of the lesson describes how an increase in substrate concentration will affect the rate of reaction when a fixed concentration of enzyme is used. Time is taken to introduce limiting factors and students will be challenged to identify substrate concentration as the limiting factor before the maximum rate is attained and then they are given discussion time to identify the possible factors after this point. A series of exam-style questions are used throughout the lesson and the mark schemes are displayed to allow the students to assess their understanding and for any misconceptions to be immediately addressed. Moving forwards, the students have to use their knowledge of substrate concentration to construct a graph to represent the relationship between enzyme concentration and rate of reaction and they have to explain the different sections of the graph and identify the limiting factors.
The final section of the lesson describes how the availability of enzymes is controlled in living organisms. Students will come to recognise that this availability is the result of enzyme synthesis and enzyme degradation and a series of tasks will introduce the details of transcription and translation and therefore prepare them for the lessons in topic 6.
Please note that this lesson explains the Biology behind the effect of concentration on enzyme-catalysed reactions and not the methodology involved in carrying out such an investigation as this is covered in a core practical lesson.
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Topic 3.2: Factors that affect enzyme action (CIE A-level Biology)
This bundle of 5 lessons covers the majority of the content in topic 3.2 of the CIE A-level Biology specification. Each lesson consists of an engaging Powerpoint with accompanying resources that cover the following points: * Explain the effects of temperature on the rate of an enzyme-catalysed reaction * Explain the effects of pH on the rate of an enzyme-catalysed reaction * Explain the effects of enzyme and substrate concentration on the rate of an enzyme-catalysed reaction * Explain the effects of inhibitor concentration on the rate of an enzyme-catalysed reaction * Explain the effects of inhibitors, both competitive and non-competitive, on the rate of enzyme activity * Explain the effect of immobilising an enzyme in alginate on its activity as compared with its activity when free in solution
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Topic 3: Enzymes (CIE A-level Biology)
This lesson bundle contains 6 fully-resourced lessons which have been designed to engage and motivate the students whilst covering the detailed content of topic 3 (Enzymes) in the CIE A-level Biology specification. These globular proteins catalyse biological reactions throughout living organisms so a deep understanding of this topic is important for all of the other 18 topics in this course. The wide range of activities that are included within the lesson PowerPoints and accompanying resources will cover the following specification points: * Enzymes are globular proteins that catalyse reactions * The mode of action of enzymes * The lock and key hypothesis and the induced-fit model * The effect of temperature on the rate of an enzyme-catalysed reaction * The effect of pH on the rate of an enzyme-catalysed reaction * The effect of enzyme and substrate concentration on the rate of an enzyme-catalysed reaction * The effect of inhibitor concentration on the rate of an enzyme-catalysed reaction * The effect of competitive and non-competitive inhibitors on enzyme activity * Immobilising an enzyme in alginate
£10.00
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