This lesson describes the role of the rER and the Golgi apparatus in the formation of proteins, the transport within cells and their secretion. The PowerPoint and accompanying resources have been designed to cover point 3.4 of the Edexcel International A-level Biology specification and also includes key details about the role of the cytoskeleton in the transport of the vesicles that contain the protein between the organelles and the membrane.
The lesson begins with the introduction of the cytoskeleton and explains how this network of protein structures transverses across the cytoplasm and is fundamental to the transport of molecules between organelles. The lesson has been planned to closely tie in with the previous lesson on the ultrastructure of eukaryotic cells and students are challenged on their knowledge of the function of the organelles involved in protein formation (and modification) through a series of exam-style questions. By comparing their answers against the mark scheme embedded in the PowerPoint, students will be able to assess their understanding of the following:
- Transcription in the nucleus to form an mRNA strand and the exit of this nucleic acid through the nuclear pore
- Translation at the ribosomes on the surface of the rER to assemble the protein
- Transport of the vesicles containing the protein to the Golgi apparatus
- Modification of the protein at the Golgi apparatus
- Formation of the Golgi vesicle and its transport to the cell membrane for exocytosis
Time is taken to discuss the finer details of this process such as the arrival of the vesicle at the cis face and the transport away from the trans face and the requirement of ATP for the transport of the vesicles along the microtubule track and exocytosis.
The remainder of the lesson uses a series of exam-style questions about digestive enzymes (extracellular proteins) to challenge the students on their recall of the structure of starch and proteins
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The ultrastructure of cells (Edexcel Int. A-level Biology)
This lesson bundle contains 6 lessons which have been designed to cover the Edexcel International A-level Biology specification points which focus on the structure of eukaryotic and prokaryotic cells and the functions of their components. The lesson PowerPoints are highly detailed, and along with the accompanying worksheets, they have been planned at length to contain a wide range of engaging tasks which cover the following A-level Biology content found in topics 2, 3 and 4 of the course: 2.2 (i): Know the structure and function of cell membranes 3.1: Know that all living organisms are made of cells, sharing some common features 3.2: Understand how the cells of multicellular organisms are organised into tissues, tissues into organs and organs into systems 3.3: Know the ultrastructure of eukaryotic cells, including nucleus, nucleolus, ribosomes, rough and smooth endoplasmic reticulum, mitochondria, centrioles, lysosomes, and Golgi apparatus 3.4: Understand the role of the rough endoplasmic reticulum (rER) and the Golgi apparatus in protein transport within cells, including their role in the formation of extracellular enzymes 3.5: Know the ultrastructure of prokaryotic cells, including cell wall, capsule, plasmid, flagellum, pili, ribosomes, mesosomes and circular DNA 3.11: Understand how mammalian gametes are specialised for their functions (including the acrosome in sperm and the zona pellucida in the egg) 4.1 (i): Know the ultrastructure of plant cells (cell walls, chloroplasts, amyloplasts, vacuole, tonoplast, plasmodesmata, pits and middle lamella) and be able to compare it with animal cells 4.1 (ii): understand the function of the structures listed in (i)
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Topic 3: Cell structure, Reproduction & Development (Edexcel International A-level Biology)
The locus and linkage, meiosis, differential gene expression and protein transport within cells lessons have been uploaded for free and by downloading these, you will be able to observe the detail of planning that has gone into all of the lessons that are included in this bundle. This intricate planning ensures that the students are engaged and motivated whilst the detailed content of topic 3 (Cell structure, Reproduction and Development) of the Edexcel International A-level Biology specification is covered. The 12 lesson PowerPoints and accompanying resources contain a wide range of activities which cover the following topic 3 specification points: * All living organisms are made of cells * Cells of multicellular organisms are organised into tissues, organs and organ systems * The ultrastructure of eukaryotic cells * The function of the organelles in eukaryotic animal cells * The role of the RER and Golgi apparatus in protein transport within cells * The ultrastructure of prokaryotic cells * Magnification and resolution in light and electron microscopes * The gene locus is the location of a gene on a chromosome * The linkage of genes on a chromosome * The role of meiosis in ensuring genetic variation * Understand how the mammalian gametes are specialised for their functions * The role of mitosis and the cell cycle in growth and asexual reproduction * The meaning of the terms stem cell, pluripotent, totipotent, morula and blastocyst * The decisions that have to be made about the use of stem cells in medical therapies * Cells become specialised through differential gene expression * One gene can give rise to more than one protein through post-transcriptional changes to mRNA * Phenotype is the interaction between genotype and the environment * Some phenotypes are affected by multiple alleles or by polygenic inheritance Due to the detail included in all of these lessons, it is estimated that it will take in excess of 6 weeks of allocated A-level teaching time to complete the teaching of the bundle
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