pdf, 1.15 MB
pdf, 1.15 MB

A full revision booklet covering all aspects of Recombinant DNA Technology for AQA Section 3.8.4.1 on how to form Recombinant DNA

Includes detailed explanations, key words, easy to follow methods and many diagrams

Content:

  • Introduction to Recombinant DNA
  • Isolation of DNA (reverse transcriptase, restriction endonucleases, sticky ends and gene machines)
  • Insertion (types of vectors, DNA ligase)
  • Transformation (Calcium ions and heat shock)
  • Identification (antibiotic resistance, enzyme and GFP marker genes)
  • Growth

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