This lesson describes and explains the effect of an increasing temperature on the rate of an enzyme-catalysed reaction. The PowerPoint and the accompanying resource are part of the 1st lesson in a series of 4 which cover the content detailed in point 3.2 (a) of the CIE A-level Biology specification and this lesson has been specifically planned to tie in with the lesson in 3.1 where the properties of enzymes and their mechanism of action were introduced.
The lesson begins by challenging the students to recognise optimum as a key term from its 6 synonyms that are shown on the board. Time is taken to ensure that the students understand that the optimum temperature is the temperature at which the most enzyme-product complexes are produced per second and therefore the temperature at which the rate of an enzyme-controlled reaction works at its maximum. The optimum temperatures of DNA polymerase in humans and in a thermophilic bacteria and RUBISCO in a tomato plant are used to demonstrate how different enzymes have different optimum temperatures and the roles of the latter two in the PCR and photosynthesis are briefly described to prepare students for these lessons in topics 19 and 13.
Moving forwards, the rest of the lesson focuses on enzyme activity at temperatures below the optimum and at temperatures above the optimum. Students will understand that increasing the temperature increases the kinetic energy of the enzyme and substrate molecules, and this increases the likelihood of successful collisions and the production of enzyme-substrate and enzyme-product complexes. When considering the effect of increasing the temperature above the optimum, continual references are made to the previous lesson and the control of the shape of the active site by the tertiary structure. Students will be able to describe how the hydrogen and ionic bonds in the tertiary structure are broken by the vibrations associated with higher temperatures and are challenged to complete the graph to show how the rate of reaction decreases to 0 when the enzyme has denatured.
Please note that this lesson has been designed specifically to explain the relationship between the change in temperature and the rate of reaction and not the practical skills that would be covered in a core practical lesson
Get this resource as part of a bundle and save up to 33%
A bundle is a package of resources grouped together to teach a particular topic, or a series of lessons, in one place.
Bundle
Topic 3.2: Factors that affect enzyme action (CIE A-level Biology)
This bundle of 5 lessons covers the majority of the content in topic 3.2 of the CIE A-level Biology specification. Each lesson consists of an engaging Powerpoint with accompanying resources that cover the following points: * Explain the effects of temperature on the rate of an enzyme-catalysed reaction * Explain the effects of pH on the rate of an enzyme-catalysed reaction * Explain the effects of enzyme and substrate concentration on the rate of an enzyme-catalysed reaction * Explain the effects of inhibitor concentration on the rate of an enzyme-catalysed reaction * Explain the effects of inhibitors, both competitive and non-competitive, on the rate of enzyme activity * Explain the effect of immobilising an enzyme in alginate on its activity as compared with its activity when free in solution
£8.50
Bundle
Topic 3: Enzymes (CIE A-level Biology)
This lesson bundle contains 6 fully-resourced lessons which have been designed to engage and motivate the students whilst covering the detailed content of topic 3 (Enzymes) in the CIE A-level Biology specification. These globular proteins catalyse biological reactions throughout living organisms so a deep understanding of this topic is important for all of the other 18 topics in this course. The wide range of activities that are included within the lesson PowerPoints and accompanying resources will cover the following specification points: * Enzymes are globular proteins that catalyse reactions * The mode of action of enzymes * The lock and key hypothesis and the induced-fit model * The effect of temperature on the rate of an enzyme-catalysed reaction * The effect of pH on the rate of an enzyme-catalysed reaction * The effect of enzyme and substrate concentration on the rate of an enzyme-catalysed reaction * The effect of inhibitor concentration on the rate of an enzyme-catalysed reaction * The effect of competitive and non-competitive inhibitors on enzyme activity * Immobilising an enzyme in alginate
£10.00
Something went wrong, please try again later.
This resource hasn't been reviewed yet
To ensure quality for our reviews, only customers who have purchased this resource can review it
Report this resourceto let us know if it violates our terms and conditions.
Our customer service team will review your report and will be in touch.
£2.00