This detailed lesson describes the role of the mRNA, tRNA, amino acids and the ribosome during the second stage of protein synthesis - translation. Both the PowerPoint and accompanying resources have been designed to cover the third part of point 4.2 of the AQA A-level Biology specification and ties in with the previous lessons in this topic on RNA and transcription. Translation is a topic which is often poorly understood and so this lesson has been written to enable the students to understand how to answer the different types of questions by knowing and including the key details of the structures involved. The lesson begins by challenging the students to consider why it is so important that the amino acids are assembled in the correct order during the formation of the chain. Moving forwards, a quick quiz round called “LOST IN TRANSLATION” is used to check on their prior knowledge of the mRNA strand, the tRNA molecules, the genetic code and the ribosomes. The next task involves a very detailed description of translation that has been divided into 14 statements which the students have to put into the correct order. By giving them a passage that consists of this considerable detail, they can pick out the important parts to use in the next task where they have to answer shorter questions worth between 3 and 4 marks. These types of questions are common in the assessments and by building up through the lesson, their confidence to answer this type should increase. The final two tasks of the lesson involve another quiz, where the teams compete to transcribe and translate in the quickest time before using all that they have learnt to answer some questions which involve the genetic code and the mRNA codon table.

This fully-resourced lesson explains how gel electrophoresis is used to analyse nucleic acids and proteins and explores its applications in forensic science and medical diagnosis. The engaging and detailed PowerPoint and accompanying resource have been written to cover point 19.1 (d) of the CIE International A-level Biology specification As a whole lesson, each step of the genetic fingerprinting process is covered but with the main focus on gel electrophoresis within this process. Students will be introduced to STRs and will come to recognise their usefulness in human identification as a result of the variability between individuals. Moving forwards, the involvement of the PCR and restriction enzymes are discussed and students are challenged on their knowledge of this process and these substances as they were encountered in a previous lesson. The main section of the lesson focuses on the use of gel electrophoresis to separate DNA fragments (as well as proteins) and the key ideas of separation due to differences in base pair length or molecular mass are discussed and explained. As well as current understanding checks, an application question involving Huntington’s disease is used to challenge their ability to apply their knowledge of the process to an unfamiliar situation. The remainder of the lesson describes how the DNA is transferred to a membrane and hybridisation probes are used to create a pattern on the X-ray film. Time has been taken to make continuous links to the previous lessons in topic 19.1 as well as those from topic 6 where DNA, RNA and protein synthesis were introduced.

This fully-resourced lesson explains how genetic fingerprinting can be used to analyse DNA fragments and explores its applications in forensic science and medical diagnosis. The engaging and detailed PowerPoint and accompanying resource have been written to cover all of point 8.4.3 of the AQA A-level Biology specification Each step of the genetic fingerprinting process is covered and time is taken to ensure that key details are understood. Students will be introduced to VNTRs and will come to recognise their usefulness in human identification as a result of the variability between individuals. Moving forwards, the involvement of the PCR and restriction enzymes are discussed and students are challenged on their knowledge of this process and these substances as they were encountered in a previous sub-topic. The main section of the lesson focuses on the use of gel electrophoresis to separate DNA fragments (as well as proteins) and the key ideas of separation due to differences in base pair length or molecular mass are discussed and explained. As well as current understanding checks, an application question involving Huntington’s disease is used to challenge their ability to apply their knowledge of the process to an unfamiliar situation. The remainder of the lesson describes how the DNA is transferred to a membrane and hybridisation probes are used to create a pattern on the X-ray film. Time has been taken to make continuous links to the previous lessons in topic 8 as well as those from topic 4 where DNA, RNA and protein synthesis were introduced.

This lesson explains how the polymerase chain reaction (PCR) is used to clone and amplify DNA fragments as part of the recombinant DNA technology process. The PowerPoint has been designed to cover point 19.1 © of the CIE International A-level Biology specification and there is a particular emphasis on the use of Taq polymerase as opposed to human DNA polymerase A quick quiz competition is used to introduce the PCR abbreviation before students are encouraged to discuss the possible identity of the enzyme involved and to recall the action of this enzyme. Students will learn that this reaction involves cyclical heating and cooling to a range of temperatures so the next part of this lesson focuses on each temperature and specifically the reasons behind the choice. Time is taken to examine the key points in detail, such as why Taq polymerase has to be used as it is not denatured at the high temperature as well as the involvement of the primers. This process is closely linked to other techniques like electrophoresis which is covered in a later lesson and ties are continuously made throughout the lesson

A fully-resourced lesson, which explores how changes to the sequence of bases on DNA may or may not alter protein structure. The engaging PowerPoint and accompanying resources have been designed to cover point 8.1 of the AQA A-level Biology specification and it builds on the knowledge gained during topic 4 when gene mutations were first introduced. A quiz runs throughout the lesson where students compete to recognise key terms from their definitions and the lesson begins with an edition of this round as they are challenged to recognise the definition for primary structure. Other terms that arise during the lesson relate to the different gene mutations, the genetic code and mutagenic agents. The focus of the lesson is to support students in their explanations of how a particular mutation can result in a change in the primary structure as well as being able to explain why a substitution mutation may not. In this way, their understanding of the degenerate and non-overlapping nature of the genetic code will be tested and any misconceptions can be addressed. The main section of the lesson covers substitution, deletion and addition mutations before translocation, inversion and duplication mutations are introduced. Links are made to the latter parts of topic 8 so students can understand how the change in the sequence of bases may disrupt gene expression. The final part of the lesson looks at a range of mutagenic agents that can increase the rate of mutation.

This fully-resourced lesson explores how projects to sequence the genomes of both simple and complex organisms can be used. Both the detailed PowerPoint and accompanying resources have been designed to cover the content of point 8.3 of the AQA A-level Biology specification. The start of the lesson describes each step of Sanger’s chain termination method and demonstrates how this method has paved the way for other projects. The use of the modified nucleotides are explained and links are made to the topic 4 and 2 where protein synthesis and DNA replication were first introduced. Students will learn how the radioactively-labelled nucleotide at the end of each fragment allows the next base to be determined. Key processes like gel electrophoresis are introduced and details provided to support the students when this is encountered in greater detail in 8.4.3. Moving forwards, the applications of sequencing in simple organisms like viruses and bacteria are explored and the students are challenged on their prior knowledge of bacterial pathogenesis and current understanding of sequencing through a series of exam-style questions. The final part of the lesson looks at the difficulties of translating genome knowledge into proteome knowledge and considers the development of automated methods. Due to the detail and extensiveness of this lesson, it is estimated that it will take in excess of 2 hours of allocated A-level teaching time to cover all of the points which have been written into the various tasks

This engaging lesson describes how the structure of the phloem enables this vascular tissue to transport organic substances in plants. Both the detailed PowerPoint and accompanying resource have been designed to cover the 3rd part of point 3.4.2 (Mass transport in plants) of the AQA A-level Biology specification. Comparative questions between the xylem and phloem are very common so the lesson begins by challenging the students to use their prior knowledge to complete the xylem column of a table with details including the presence of lignin and bordered pits and specific substances which are transported in this tissue. This has been written into the lesson to encourage the students to start to think about how the structure and function of the phloem may compare. 3 quiz rounds have been included in the lesson to maintain motivation and to introduce key terms. The first of these rounds will challenge the students to be the first to recognise descriptions of sucrose and amino acids as they learn that these are the two most common assimilate, which are the substances transported by the phloem. The focus of this lesson is the relationship between structure and function and all descriptions have these two parts highlighted to support the students to recognise the link. Moving forwards, students will be introduced to the sieve tube elements and the companion cells and time is taken to consider why the structure of these cells are so different. Current understanding checks are interspersed throughout the lesson to ensure that any misconceptions can be quickly addressed. The plasmodesmata is described to allow students to understand how assimilates move from the companion cells to the sieve tube elements as this will be particularly important for the next lesson on translocation. The final task of the lesson challenges the students to write a detailed passage about the structure and function of the phloem, incorporating all of the information that they have absorbed throughout the course of the lesson.

This fully-resourced lesson describes how the structure of the xylem tissue allows water to be transported in the stem and leaves. Written for AQA A-level Biology, the engaging and detailed PowerPoint and the accompanying worksheets cover the 1st part of specification point 3.4.2 (mass transport in plants) and includes a detailed description of the cohesion-tension theory. The first part of the lesson focuses on the relationship between the structure and function of the xylem tissue. A number of quiz competitions have been included in the lesson to maintain engagement and to introduce key terms. The 1st round does just that and results in the introduction of lignin which leads into the explanation of how the impregnation of this substance in the cell walls result in the death and subsequent decay of the cell structures. Students are encouraged to discuss how the formation of this hollow tube enables the transport of water to be effective. Moving forwards, other structures such as the bordered pits are introduced and an understanding of their function is tested later in the lesson. The remainder of the lesson focuses on the transport of water in the stem and leaves by root pressure and the transpiration pull, which includes cohesion, tension and adhesion. The lesson has been designed to make links to information covered earlier in the lesson as well to topics from earlier in the specification such as cell structures and biological molecules Due to the extensiveness of this lesson, it is estimated that it will take in excess of 2/3 A-level teaching hours to cover the detail included in this lesson.

This fully-resourced lesson describes how the mechanisms of root pressure and transpiration pull move water upwards in the xylem to the leaves. The detailed PowerPoint and accompanying, differentiated resources have primarily been designed to cover the second part of point 7.2 [c] of the CIE International A-level Biology specification but also cover 7.2 [b] as the cohesion-tension theory and adhesion are described and explained. This lesson has been written to follow on from the end of the previous lesson, which finished with the description of the transport of the water and mineral ions from the endodermis to the xylem. Students are immediately challenged to use this knowledge to understand root pressure and the movement by mass flow down the pressure gradient. Moving forwards, time is taken to study the details of transpiration pull and the interaction between cohesion, tension and adhesion in capillary action is explained. Understanding is constantly checked through a range of tasks and prior knowledge checks are also written into the lesson to challenge the students to make links to previously covered topics such as the structure of the transport tissues. The final part of the lesson considers the journey of water through the leaf and ultimately out of the stomata in transpiration. A step by step guide using questions to discuss and answer as a class is used to support the students before the final task challenges them to summarise this movement through the leaf.

This detailed lesson describes the transport of water into the plant as well as the movement across the cortex to the endodermis and to the xylem. Both the engaging PowerPoint and accompanying resource have been designed to cover the first part of point 3.1.3 (d) as detailed in the OCR A-level Biology A specification. The lesson begins by looking at the specialised features of the root hair cell so that students can understand how these epidermal cells absorb water and mineral ions from the soil. Moving forwards, students are introduced to key terminology such as epidermis and root cortex before time is taken to look at the symplast, vacuolar and apoplast pathways that water and minerals use to transverse the cortex. Discussion points are included throughout the lesson to encourage the students to think about each topic in depth and challenges them to think about important questions such as why the apoplast pathway is needed for the water carrying the ions. The main part of the lesson focuses on the role of the endodermis in the transport of the water and ions into the xylem. Students will be introduced to the Casparian strip and will learn how this layer of cells blocks the apoplast pathway. A step by step method using class questions and considered answers is used to guide them through the different steps and to support them when writing the detailed description. This lesson has been specifically written to tie in with the next lesson on the pathways and mechanisms by which water and mineral ions are transported to the leaves and then out into the air surrounding the leaves.

This engaging lesson describes the relationship between the structure and properties of a phopholipid and explains the link to its role in membranes. The PowerPoint has been designed to cover the second part of point 1.3 of the AQA A-level Biology specification and includes constant references to the previous lesson on triglycerides. The role of a phospholipid in a cell membrane provides the backbone to the whole lesson. A quick quiz round called FAMILY AFFAIR, challenges the students to use their knowledge of the structure of a triglyceride to identify the shared features in a phospholipid. This then allows the differences to be introduced, such as the presence of a phosphate group in place of the third fatty acid. Moving forwards, the students will learn that the two fatty acid tails are hydrophobic whilst the phosphate head is hydrophilic which leads into a key discussion point where the class has to consider how it is possible for the phospholipids to be arranged when both the inside and outside of a cell is an aqueous solution. The outcome of the discussion is the introduction of the bilayer which is critical for the lesson in topic 2 on the fluid mosaic model. The final part of the lesson explains how both facilitated diffusion and active transport mean that proteins are found floating in the cell membrane and this also helps to briefly prepare the students for upcoming topic 2 lessons.

This detailed lesson describes the structure of a nucleotide including the structure of the phosphorylated nucleotide, ATP. The engaging PowerPoint has been designed to cover point (a) of topic 6.1 as detailed in the CIE International A-level Biology specification and links are made throughout to earlier topics such as biological molecules as well as to upcoming topics like DNA structure and replication. Students were introduced to the term monomer and nucleotide in topic 2, so the start of the lesson challenges them to recognise this latter term when only the letters U, C and T are shown. This has been designed to initiate conversations about why only these letters were used so that the nitrogenous bases can be discussed later in greater detail. Moving forwards, students will learn that a nucleotide is the monomer to a polynucleotide and that deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are two examples of this type of polymer. The main part of the lesson has been filled with various tasks that explore the structural similarities and structural differences between DNA and RNA. This begins by describing the structure of a nucleotide as a phosphate group, a pentose sugar and a nitrogenous base. Time is taken to consider the details of each of these three components which includes the role of the phosphate group in the formation of a phosphodiester bond between adjacent nucleotides on the strand. At this point students are challenged on their understanding of condensation reactions and have to identify how the hydroxyl group associated with carbon 3 is involved along with the hydroxyl group of the phosphoric acid molecule. A number of quiz rounds are used during this lesson, as a way to introduce key terms in a fun and memorable way. One of these rounds introduces adenine and guanine as the purine bases and thymine, cytosine and uracil as the pyrimidine bases and the students are shown that their differing ring structures can be used to distinguish between them. The remainder of the lesson focuses on ATP as a phosphorylated nucleotide and links are made to the hydrolysis of this molecule for energy driven reactions in cells such as active transport

This fully-resourced lesson describes the process of DNA replication and explains how this ensures genetic continuity between generations. Both the detailed PowerPoint and accompanying resources have been designed to cover point 1.5.2 of the AQA A-level Biology specification and also explains why it is known as semi-conservative. The main focus of this lesson is the roles of DNA helicase in the breaking the hydrogen bonds between nucleotide bases and DNA polymerase in forming the growing nucleotide strands. Students are also introduced to DNA ligase to enable them to understand how this enzyme functions to join the nucleic acid fragments. Time is taken to explain key details, such as the assembly of strands in the 5’-to-3’ direction, so that the continuous manner in which the leading strand is synthesised can be compared against that of the lagging strand. The students are constantly challenged to make links to previous topics such as DNA structure and hydrolysis reactions through a range of exam questions and answers are displayed so that any misconceptions are quickly addressed. The main task of the lesson asks the students to use the information provided in the lesson to order the sequence of events in DNA replication before discussing how the presence of a conserved strand and a newly built strand in each new DNA molecule shows that it is semi-conservative.

This detailed and fully-resourced lesson describes the relationship between the structure, properties and functions of glycogen, starch and cellulose. The engaging PowerPoint and accompanying resources have been designed to cover specification points 2.1.2 (f) & (g) of the OCR A-level Biology A course and continual links are also made to the previous lessons in this topic where the monosaccharides and disaccharides were introduced. The lesson begins with the CARBOHYDRATE WALL where students have to use their prior knowledge to collect the 9 carbohydrates on show into 3 groups. This results in glycogen, starch and cellulose being grouped together as polysaccharides and the structure, properties and functions of these large carbohydrates are covered over the course of the lesson. Students will learn how key structural features like the 1 - 4 and 1 - 6 glycosidic bonds and the hydrogen bonds dictate whether the polysaccharide chain is branched or unbranched and also dictate whether the chain spirals or not. Following the description of the structure of glycogen, students are challenged to design an exam question in the form of a comparison table so that it can be completed as the lesson progresses and they learn more about starch and cellulose. This includes a split in the starch section of the table so that the differing structures and properties of amylose and amylopectin can be considered. The importance of the compact structure for storage is discussed as well as the branched chains of amylopectin acting as quick source of energy when it is needed. In the final part of the lesson, time is taken to focus on the hydrogen bonds between rotated glucose molecules on the same chain and between different chains and to explain how the formation of cellulose microfibrils and macrofibrils provides plant cells with the additional strength needed to support the whole plant. Due to the detail included in this lesson, it is estimated that it will take in excess of 2 hours of allocated teaching time to complete