This fully-resourced lesson explores how other respiratory substrates, such as lipids and proteins, can be used to produce molecules of ATP. The PowerPoint and accompanying resources have been designed to cover the 7th and final part of point 5.2 of the AQA A-level Biology specification which states that students should know how these substrates enter the Krebs cycle. This lesson has been written to challenge the knowledge of the earlier parts of the topic of respiration and so contains constant prior knowledge checks which come in a range of forms. Students will learn that lipids and proteins can be used as respiratory substrates and will recognise the different ways that they enter the respiratory pathway. Time is taken to look at the beta oxidation pathway and again students are challenged to compare the products of this pathway against that of the Link reaction.

This fully-resourced lesson looks at the series of oxidation-reduction reactions that form the Krebs cycle and focuses on the products in terms of reduced NAD, FAD and ATP. The engaging PowerPoint and accompanying resource have both been designed to cover the fifth part of point 5.2 of the AQA A-level Biology specification. The lesson begins with a version of the Impossible game where students have to spot the connection between 8 of the 9 terms and will ultimately learn that this next stage is called the Krebs cycle. The main part of the lesson challenges the students to use descriptions of the main steps of the cycle to continue their diagram of the reactions. Students are continually exposed to key terminology such as decarboxylation and dehydrogenation and they will learn where carbon dioxide is lost and reduced NAD and FAD are generated. They will also recognise that ATP is synthesised by substrate level phosphorylation. The final task challenges them to apply their knowledge of the cycle to work out the numbers of the different products and to calculate the number of ATP that must be produced in the next stage This lesson has been designed to tie in with the other uploaded lessons on glycolysis, anaerobic respiration, the Link reaction and oxidative phosphorylation.

This detailed lesson introduces the four stages of aerobic respiration and looks at the relationship between structure and function of the mitochondrion. The engaging PowerPoint and accompanying resource have been designed to cover points 12.2 (a) and (i) of the CIE International A-level Biology specification which states that students should be able to demonstrate and apply an understanding of the inner and outer mitochondrial membranes, cristae, matrix and mitochondrial DNA. The lesson begins with an introduction to glycolysis and students will learn how this first stage of aerobic respiration is also the first stage when oxygen isn’t present. A version of “GUESS WHO” challenges students to use a series of structural clues to whittle the 6 organelles down to just the mitochondrion so that they can learn how the other three stages take place inside this organelle. Moving forwards, the key components of the organelle are identified on a diagram. Students are introduced to the stages of respiration so that they can make a link to the parts of the cell and the mitochondria where each stage occurs. Students will learn that the presence of decarboxylase and dehydrogenase enzymes in the matrix along with coenzymes and oxaloacetate allows the Link reaction and the Krebs cycle to run. Finally, time is taken to introduce the electron transport chain and the enzyme, ATP synthase, so that students can begin to understand how the flow of protons across the inner membrane results in the production of ATP.

This fully-resourced lesson explores how glucose as well as the other respiratory substrates, such as lipids and proteins, can enter the respiratory pathway and therefore can be respired to produce molecules of ATP. The engaging PowerPoint and accompanying resources have been designed to cover points 12.1 (f) and (g) of the CIE International A-level Biology specification which states that students should be able to explain the relative energy values of carbohydrates, lipids and proteins and be able to determine respiratory quotients from equations. This lesson has been written to challenge current understanding as well as introduce details of glycolysis, the link reaction and Krebs cycle as these stages have yet to be covered fully. Students will learn that lipids and proteins can be used as respiratory substrates and will recognise the different ways that they enter the respiratory pathway. A quick quiz competition is used to introduce the relative energy value for carbohydrates and students are challenged to predict how the values for lipids and proteins will compare. As a result, students will recognise that a greater number of hydrogen atoms results in a greater availability of protons to form the proton gradient to fuel the production of ATP. The rest of the lesson focuses on the calculation of the respiratory quotient and time is taken to look at how the result can be interpreted to determine which substrates were respired.

This engaging lesson looks at the structure of the quaternary protein, haemoglobin, and describes its role with red blood cells in the transport of oxygen. The PowerPoint has been designed to cover the first part of point 3.4.1 of the AQA A-level Biology specification and explains how the cooperative nature of binding results in a loading of each molecule with 4 oxygen molecules and describes how it is unloaded at the respiring cells too. The lesson begins with a version of the quiz show Pointless to introduce haemotology as the study of the blood conditions. Students are told that haemoglobin has a quaternary structure and are challenged to use their prior knowledge of biological molecules to determine what this means for the protein. They will learn that each of the 4 polypeptide chains contains a haem group with an iron ion attached and that it is this group which has a high affinity for oxygen. Time is taken to discuss how this protein must be able to load (and unload) oxygen as well as transport the molecules to the respiring tissues. Students will plot the oxyhaemoglobin dissociation curve and the S-shaped curve is used to encourage discussions about the ease with which haemoglobin loads each molecule. Students will learn that a conformational change upon binding of the first oxygen leads to it being easier to bind future oxygens and that this is known as cooperative binding. This lesson has been written to tie in with the other uploaded lesson on the Bohr effect.

This fully-resourced lesson explores how pyruvate can be converted to lactate or ethanol using reduced NAD and that the reoxidation of the coenzyme allows glycolysis to continue. The engaging and detailed PowerPoint and accompanying differentiated resources have been designed to cover points 12.2 (j) and (k) of the CIE International A-level Biology specification which states that students should be able to explain the production of a small yield of ATP in anaerobic conditions and recognise the concept of an oxygen debt The lesson begins with a focus on the coenzyme, NAD, and students are challenged to recall details of its role in the oxidation of triose phosphate. Students will learn that oxidative phosphorylation in aerobic respiration allows these coenzymes to be reoxidised but that another metabolic pathway has to operate when there is no oxygen. Time is taken to go through the lactate and ethanol fermentation pathways and students are encouraged to discuss the conversions before applying their knowledge to complete diagrams and passages about the pathways. Understanding checks in a range of forms are used to enable the students to assess their progress whilst prior knowledge checks allow them to recognise the links to earlier topics. Students will also be introduced to the oxygen debt and will learn how the volume consumed after vigorous exercise is used to catabolise lactic acid and restore the body’s stores to normal levels. This lesson has been written to tie in with the other uploaded lessons on the stages of aerobic respiration.

This fully-resourced lesson explains how gene mutations can occur by substitution, deletion and insertion and explores how these base pair changes can affect the primary structure of the polypeptide and therefore the phenotype. The engaging and detailed PowerPoint and accompanying resources have been designed to cover point 16.2 (e) of the CIE International A-level Biology specification which states that students should understand how these mutations occur and can affect the phenotype. In order to understand how a change in the base sequence can affect the order of the amino acids, students must be confident in their understanding and application of protein synthesis which was taught in topic 6. Therefore, the start of the lesson focuses on transcription and translation and students are guided through the use of the codon table to identify amino acids. Moving forwards, a quick quiz competition is used to introduce the names of three types of gene mutation whilst challenging the students to recognise terms which are associated with the genetic code and were met in the previous lesson. The main focus of the lesson is base substitutions and how these mutations may or may not cause a change to the amino acid sequence. The students are challenged to use their knowledge of the degenerate nature of the genetic code to explain how a silent mutation can result. The rest of the lesson looks at base deletions and base insertions and students are introduced to the idea of a frameshift mutation. One particular task challenges the students to evaluate the statement that base deletions have a bigger impact on primary structure than base substitutions. This is a differentiated task and they have to compare the fact that the reading frame is shifted by a deletion against the change in a single base by a substitution

This detailed lesson describes and explains the blood pressure changes that occur during systole and diastole of the cardiac cycle. The PowerPoint and accompanying resource have been designed to cover point 8.2 © of the CIE International A-level Biology specification. The start of the lesson introduces the cardiac cycle as well as the key term systole, so that students can immediately recognise that the three stages of the cycle are atrial and ventricular systole followed by diastole. Students are challenged on their prior knowledge of the structure of the heart as they have to name and state the function of an atrioventricular and semi-lunar valve from an internal diagram. This leads into the key point that pressure changes in the chambers and the major arteries results in the opening and closing of these sets of valves. Students are given a description of the pressure change that results in the opening of the AV valves and shown where this would be found on the graph detailing the pressure changes of the cardiac cycle. They then have to use this as a guide to write descriptions for the closing of the AV valve and the opening and closing of the semi-lunar valves and to locate these on the graph. By providing the students with this graph, the rest of the lesson can focus on explaining how these changes come about. Students have to use their current and prior knowledge of the chambers and blood vessels to write 4 descriptions that cover the cardiac cycle. The final part of the lesson covers the changes in the volume of the ventricle. This lesson has been written to tie in with the other uploaded lessons on the heart as detailed in topic 8.2

This fully-resourced REVISION lesson has been written to challenge the students on their knowledge of the content of topic 1 (Cell structure) of the CIE International A-level Biology specification. The PowerPoint and accompanying resources will motivate the students whilst they assess their understanding of the content and identify any areas which may require further attention. The wide range of activities have been written to cover as much of the topic as possible but the following specification points have been given particular focus: ATP is produced in mitochondria and chloroplasts and the role of ATP in cells Recognising eukaryotic cell structures and outlining their functions Calculating actual sizes from electron micrographs The structural features of a typical prokaryotic cell The key features of viruses as non-cellular structures Distinguish between resolution and magnification Quiz rounds such as “GUESS WHO of CELL STRUCTURES” and “YOU DO THE MATH” are used to test the students on the finer details of their knowledge of the structure and functions of the organelles and some key numerical facts

This fully-resourced lesson looks at the cardiac cycle and relates the structure and operation of the mammalian heart to its function. The engaging and detailed PowerPoint and accompanying resources have been designed to cover point 1.4 (i) of the Pearson Edexcel A-level Biology A (Salters Nuffield) specification As the structure of the heart was covered at GCSE, the lesson has been planned to build on this prior knowledge whilst adding the key details which will enable students to provide A-level standard answers. The primary focus is the identification of the different structures of the heart but it also challenges their ability to recognise the important relationship to function. For example, time is taken to ensure that students can explain why the atrial walls are thinner than the ventricular walls and why the right ventricle has a thinner wall than the left ventricle. Opportunities are taken throughout the lesson to link this topic to the others found in topic 1 including those which have already been covered like circulatory systems as well as those which are upcoming such as the initiation of heart action. There is also an application question where students have to explain why a hole in the ventricular septum would need to be repaired if it doesn’t naturally close over time. The next part of the lesson introduces the cardiac cycle as well as the key term systole, so that students can immediately recognise that the three stages of the cycle are atrial and ventricular systole followed by cardiac diastole. Students are challenged to name and state the function of an atrioventricular and semi-lunar valve from an internal diagram. This leads into the key point that pressure changes in the chambers and the major arteries results in the opening and closing of these sets of valves. Students are given a description of the pressure change that results in the opening of the AV valves and shown where this would be found on the graph detailing the pressure changes of the cardiac cycle. They then have to use this as a guide to write descriptions for the closing of the AV valve and the opening and closing of the semi-lunar valves and to locate these on the graph. By providing the students with this graph, the rest of the lesson can focus on explaining how these changes come about. Students have to use their current and prior knowledge of the chambers and blood vessels to write 4 descriptions that cover the cardiac cycle. The final part of the lesson covers the changes in the volume of the ventricle. It is estimated that it will take in excess of 2 hours of allocated A-level teaching time to cover the detail included in this lesson as required by this specification point

This detailed lesson explains how the process of transcription results in the production of messenger RNA (mRNA). Both the detailed PowerPoint and accompanying resource have been designed to specifically cover the first part of point 6.2 (d) of the CIE International A-level Biology specification. The lesson begins by challenging the students to recall that most of the nuclear DNA in eukaryotes does not code for polypeptides. This allows the promoter region and terminator region to be introduced, along with the structural gene. Through the use of an engaging quiz competition, students will learn that the strand of DNA involved in transcription is known as the template strand and the other strand is the coding strand. Links to previous lessons on DNA and RNA structure are made throughout and students are continuously challenged on their prior knowledge as well as they current understanding of the lesson topic. Moving forwards, the actual process of transcription is covered in a 7 step bullet point description where the students are asked to complete each passage using the information previously provided. An exam-style question is used to check on their understanding before the final task of the lesson looks at the journey of mRNA to the ribosome for the next stage of translation. This lesson has been written to challenge all abilities whilst ensuring that the most important details are fully explained.